Select Publications

Our lab publishes at the top of both our fields:



Edward N. Schmidt, Xue Yan Guo, Duong T. Bui, Jaesoo Jung, John S. Klassen, Matthew S. Macauley, Dissecting the abilities of murine Siglecs to interact with gangliosides, Journal of Biological Chemistry, 2024, 107482, ISSN 0021-9258,

Eskandari-Sedighi G, Crichton M, Zia S, Gomez-Cardona E, Cortez LM, Patel ZH, Takahashi-Yamashiro K, St Laurent CD, Sidhu G, Sarkar S, Aghanya V, Sim VL, Tan Q, Julien O, Plemel JR, Macauley MS. Alzheimer’s disease associated isoforms of human CD33 distinctively modulate microglial cell responses in 5XFAD mice. Mol Neurodegener. 2024 May 27;19(1):42. doi: 10.1186/s13024-024-00734-8. PMID: 38802940; PMCID: PMC11129479.

Kelli A. McCord, Chao Wang, Mirjam Anhalt, Wayne W. Poon, Amanda L. Gavin, Peng Wu, and Matthew S. Macauley. Dissecting the Ability of Siglecs To Antagonize Fcγ Receptors. ACS Central Science Article ASAP DOI: 10.1021/acscentsci.3c00969


Tijaro-Bulla S, Osman EA, St Laurent CD, McCord KA, Macauley MS, Gibbs JM. Disrupting Protein Expression with Double-Clicked sgRNA-Cas9 Complexes: A Modular Approach to CRISPR Gene Editing. ACS Chem Biol. 2023 Oct 20;18(10):2156-2162. doi: 10.1021/acschembio.3c00140. Epub 2023 Aug 9. PMID: 37556411.

Schmidt EN, Lamprinaki D, McCord KA, Joe M, Sojitra M, Waldow A, Nguyen J, Monyror J, Kitova EN, Mozaneh F, Guo XY, Jung J, Enterina JR, Daskhan GC, Han L, Krysler AR, Cromwell CR, Hubbard BP, West LJ, Kulka M, Sipione S, Klassen JS, Derda R, Lowary TL, Mahal LK, Riddell MR, Macauley MS. Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket. Nat Commun. 2023 Apr 22;14(1):2327. doi: 10.1038/s41467-023-38030-6. PMID: 37087495; PMCID: PMC10122656.


Hardy LC, Smeekens JM, Raghuwanshi D, Sarkar S, Daskhan GC, Rogers S, Nycholat C, Maleki S, Burks AW, Paulson JC, Macauley MS, Kulis MD. Targeting CD22 on memory B cells to induce tolerance to peanut allergens. J Allergy Clin Immunol. 2022 Dec;150(6):1476-1485.e4. doi: 10.1016/j.jaci.2022.06.022. Epub 2022 Jul 14. PMID: 35839842; PMCID: PMC9813968.

Park H, Osman EA, Cromwell CR, St Laurent CD, Liu Y, Kitova EN, Klassen JS, Hubbard BP, Macauley MS, Gibbs JM. CRISPR-Click Enables Dual-Gene Editing with Modular Synthetic sgRNAs. Bioconjug Chem. 2022 May 18;33(5):858-868. doi: 10.1021/acs.bioconjchem.2c00106. Epub 2022 Apr 18. PMID: 35436106.

Enterina JR, Sarkar S, Streith L, Jung J, Arlian BM, Meyer SJ, Takematsu H, Xiao C, Baldwin TA, Nitschke L, Shlomchick MJ, Paulson JC, Macauley MS. Coordinated changes in glycosylation regulate the germinal center through CD22. Cell Rep. 2022 Mar 15;38(11):110512. doi: 10.1016/j.celrep.2022.110512. PMID: 35294874; PMCID: PMC9018098.


Nguyen L$, McCord KA$, Bui TB, Bouwman KM, Kitova EN, Kumawat DDaskhan GC, Tomris I, Han L, Chopra P, Yan TJ, Willow SD, Mason AL, Lowary TL, West LJ, Hsu STD, Tompkins SM, Boons GJ, de Vries RP, Macauley MS*, Klassen JS*. Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2. In Press at Nature Chemical Biology.

Jung J, Enterina JR, Bui DT, Mozaneh F, Lin PH, Nitin, Kuo CW, Rodrigues E, Bhattacherjee A, Raeisimakiani P, Daskhan GC, St. Laurent CD, Khoo KH, Mahal LK, Zandberg WF, Huang X, Klassen JS, Macauley MS. Carbohydrate sulfation as a mechanism for fine-tuning Siglec ligands. In Press at ACS Chemical Biology.

Bhattacherjee A, Daskhan GC, Bains A, Watson EAS, Eskandari-Sedighi G, St. Laurent CD, Voronova A, Macauley MS. (2021) Increasing phagocytosis of microglia through targeting CD33 with liposomes displaying glycan ligands. Journal of Controlled Release. 338:680-693.

Hong S, Yu C, Shi Y, Wang P, Chapla DG, Rodrigues E, Moremen KW, Paulson JC, Macauley MS, Wu P. (2021) Modulation of Siglec-7 Signaling via in situ Created High-Affinity cis Ligands. ACS Central Science. 7(8):1338-1346.

Sojitra M, Sarkar S, Maghera J, Rodrigues E, Carpenter E, Seth S, Ferrer Vinals D, Bennett N, Reddy R, Khalil A, Xue X, Bell M, Zheng RB, Zhang P, Nycholat C, Ling CC, Lowary TL, Paulson JC, Macauley MS, Derda R. (2021) Genetically Encoded, Multivalent Liquid Glycan Array (LiGA). Nature Chemical Biology. 17(7):806-816.

Bhattacherjee AJung JZia S, Ho MEskandari-Sedighi GSt Laurent CD, McCord KABains ASidhu G, Sarkar S, Plemel JRMacauley MS. (2021) The CD33 short isoform is a gain-of-function variant that enhances Aβ 1-42 phagocytosis in microglia Molecular Neurodegeneration. 16(1):19.


Park H, Jaesoo J, Rodrigues E, Kitova E, Macauley MS, Klassen JS. (2020) Mass Spectrometry-based Shotgun Glycomics for Discovery of Natural Ligands of Glycan-binding Proteins. Analytical Chemistry. 92(20): 14012-14020.

Rodrigues E, Jung J, Park H, Loo C, Soukhtehzari S, Kitova EN, Mozaneh F, Daskhan G, Schmidt EN, Aghanya V, Sarkar S, Streith L, St. Laurent C, Nguyen L, Julien JP, West LJ, Williams KC, Klassen JS, and Macauley MS. (2020) A Versatile Soluble Siglec Scaffold for Sensitive and Quantitative Detection of Glycan Ligands. Nature Communications. 11(1):5091.

Movsisyan LD and Macauley MS. (2020) Structural Advances of Siglecs: Insight into Synthetic Glycan Ligands for Immunomodulation. Organic & Biomolecular Chemistry. 18(30):5784-5797.

Li Z, Kitov PI, Kitova EN, Mozenah F, Rodrigues E, Chapla DG, Moremen KW, Macauley MS, Klassen JS. CUPRA-ZYME: An assay for measuring carbohydrate-active enzyme activities, pathways and substrate specificities. Analytical Chemistry92(4):3228-3236.


Bhattacherjee A, Rodrigues E, Jung J, Luzentales-Simpson M, Enterina JR, Galleguillos D, St. Laurent CD, Nakhaei-Njad M, Fuchsberger FF, Streith L, Wang Q, Kawasaki N, Duan S, Bains A, Paulson JC, Rademacher C, Giuliani F, Sipione S, Macauley MS.Repression of Phagocytosis by Human CD33 is not Conserved with Mouse CD33. Communications Biology. 2, 450.

Enterina J, Jung J, Macauley MS. (2019) Coordinated roles for glycans in regulating the inhibitory function of CD22 on B cells. Biomedical Journal. 42(2):218-232.

Kitov PI, Kitova EN, Han L, Li Z, Jung J, Rodrigues R, Hunter CD, Cairo CW, Macauley MS, Klassen JS. (2019) A quantitative and high-throughput mass spectrometry-based glycan library screening method. Communications Biology2:268.

Natoni A, Farrell ML, Harris S, Falanx C, Kirkham-Mccarthy, Macauley MS, Reagan MR, O’Dwyer M. (2019) Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model. HeamatologicaIn Press.Link:

Bednar KJ, Nycholat CM, Rao TS, Paulson JC, Fung-Leung WP, Macauley MS. (2019) Exploiting CD22 to Selectively Tolerize Autoantibody Producing B-cells in Rheumatoid Arthritis. ACS Chemical Biolology. 14(4):644-654.


Bednar KF, Hardy L, Smeekens J, Raghuwanshi D, Duan S, Kulis MD, Macauley MS. (2018) Antigenic Liposomes for Generation of Disease-specific Antibodies. Journal of Visualized Experiments. 140.Link:

Rodrigues E and Macauley MS. (2018) Hypersialylation in Cancer: Modulation of Inflammation and Therapeutic Opportunities. Cancers. 18;10(6).

Briard JG, Jiang H, Moremen KW, Macauley MS*, Wu P*. (2018) Cell-based glycan arrays for probing glycan-glycan binding protein interactions. Nature Communications. 28;9(1):880. * co-corresponding authors

Spiller F, Nycholat CM, Kikuchi C, Paulson JC and Macauley MS. (2018) Murine red blood cells lack ligands for B cell Siglecs, allowing strong activation by erythrocyte surface antigens. Journal of Immunology200(3):949-956.

Bednar KJ, Shanina E, Ballet Ballet, Connors EP, Duan S, Juan J, Arlian BM, Kulis MD, Butcher EC, Fung-Leung WP, Rao TS, Paulson JC, and Macauley MS. (2017) Human CD22 Inhibits Murine B-cell Receptor Activation in a Human CD22 Transgenic Mouse Model. Journal of Immunology. 199(9): 3116-3128.


Orgel KA, Duan S, Wright BL, Maleki SJ, Wolf JC, Vickery BP, Burks AW, Paulson JC, Kulis MD, Macauley MS. (2017) Exploiting CD22 on Antigen-Specific B-Cells to Prevent Allergy to the Major Peanut. Journal of Allergies and Clinical Immunology. 6749(16): 30799-0.

Lopez Aguilar A, Briard JG, Yang L, Ovryn B, Macauley MS, Wu P (2017) Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling. ACS Chemical Biology12(3):611-621Link:


Territorial Acknowledgement

The University of Alberta, its buildings, labs, and research stations are primarily located on the traditional territory of Cree, Blackfoot, Métis, Nakota Sioux, Iroquois, Dene, and Ojibway/Saulteaux/Anishinaabe nations; lands that are now known as part of Treaties 6, 7, and 8 and homeland of the Métis. The University of Alberta respects the sovereignty, lands, histories, languages, knowledge systems, and cultures of First Nations, Métis and Inuit nations.

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